Genotype and hormonal effects on callus formation and regeneration in mulberry     

B. N. Susheelamma  K. Raja Shekar  A. Sarkar  M. R. Rao  R. K. Datta 《Euphytica》1996,90(1):25-29

Summary Twenty-five mulberry genotypes were studied for callus induction, to evaluate the effectiveness of hormones in promoting callus growth and to identify genotypes capable of regenerating plants. Fifteen genotypes showed callus initiation. Genotypic variation was also noted for longevity and rate of growth of callus cultures. Calli of different genotypes were maintained for more than one year. Frequency of callus initiation was high on Murashige & Skoog's modified medium incorporated with 2.0 mg/l 2,4-D, 100 mg/l casein acid hydrolysate and 150 ml/l coconut water. Regeneration through organogenesis was achieved in six genotypes indicating genotypic specificity.  相似文献   

Inheritance of Dry Root Rot (Rhizoctonia bataticola) Resistance in Chickpea (Cicer arietinum)     

P. K. Ananda  Rao M. P. Haware 《Plant Breeding》1987,98(4):349-352

The inheritance of resistance to dry root rot of chickpea caused by Rhizoctonia bataticola was studied. Parental F₁ and F₂ populations of two resistant and two susceptible parents, along with 49 F₁ progenies of one of the resistant × susceptible crosses were rested for their reaction to dry root rot using the blotting-paper technique. All F, plants of the resistant × susceptible crosses were resistant; the F₂ generation fitted a 3 resistant: 1 susceptible ratio indicating monogenic inheritance, with resistance dominant over susceptibility. F³ family segregation data confirmed the results. No segregation occurred among the progeny of resistant × resistant and susceptible × susceptible crosses.  相似文献   

Implications for dosing regimen of enrofloxacin administered concurrently with dexamethasone in febrile buffalo calves     

Sidhu  Pritam K.  Ramalingam  Balaje  Kaur  Gurpreet  Rampal  Satyavan  Coetzee  Johann F. 《Tropical animal health and production》2020,52(3):1093-1102

Tropical Animal Health and Production - The objective of the study was to determine the influence of dexamethasone (DXM) on pharmacokinetics (PK) and pharmacodynamics (PD) of enrofloxacin (ENR) for...  相似文献   

Endogenous fat losses and true and apparent fat digestibility in adult and growing dogs fed diets containing poultry offal fat     

Tabyta T. Sabchuk  Larissa W. Risolia  Camilla M. M. Souza  Ananda P. Félix  Alex Maiorka  Simone G. Oliveira 《Journal of animal physiology and animal nutrition》2020,104(6):1927-1937

The objective of this study was to evaluate the coefficient of total tract apparent digestibility (CTTAD) of dietary nutrients, endogenous fat loss (EFL), dietary metabolizable energy (ME) content, coefficient of true digestibility (CTD) of fat and faecal parameters of dogs. A total of 16 puppies and 8 adult dogs were distributed according to a double Latin square experimental design. A basal diet was formulated, to which increasing levels (60, 120 and 180 g/kg) of poultry offal fat oil were added. EFL was estimated using hyperbolic and linear regression as a function of LW/day and dry matter (DM) intake, respectively, and the CTD of fat was calculated. The CTTAD of acid-hydrolysis ether extract (AHEE) and ME content increased as dietary poultry offal fat level increased both in adult dogs and in puppies (p < .05). The CTTAD of DM and crude protein were higher in adult dogs than in puppies. EFL was estimated by hyperbolic regression as y = 98.1094 − 13.6202/x and y = 99.1280 − 26.1366/x, where y = CTTAD of AHEE (%) and x = AHEE intake/kg BW, for adult dogs and puppies, respectively, which yielded EFL values of 138 and 262 mg AHEE per kg BW per day for adult dogs and puppies respectively. The EFL estimated by linear regression was 7.89 g and 8.97 g/kg DM intake for adult dogs and puppies respectively. The CTD estimated by both methodologies was higher in puppies than in adults (p < .05). Lower faecal DM and score, and higher ammonia production were obtained in puppies than adult dogs (p < .05). The results indicate good fat utilization and that fat digestibility is underestimated, as the CTTAD of AHEE does not take into account EFL, particularly at low-fat inclusion levels and in growing dogs, which have greater EFL than adult dogs.  相似文献   

Anti-viral activity of methyl 1-chloro-7-methyl-2-propyl-1h-benzo[d] imidazole-5-carboxylate against white spot syndrome virus in freshwater crab (Paratelphusa hydrodromous)     

Karthikeyan  K.  Kumar  A. T. Manish  Harshini  D.  Pooja  G.  Daniel  J. Arul  Yasam  Pavani  Gracevictoria  G.  Priayanka  M.  Krishnaraj  P.  Vidya  R.  Devi  S. Asha  Reddy  Sabbasani Rajasekhara  Sudhakaran  Raja 《Aquaculture International》2022,30(2):989-998

Shrimp farming industries are subjected to severe economic loss due to a disease called white spot syndrome, a viral disease caused by white spot syndrome virus (WSSV) in penaeid shrimp. Numerous active compounds in the market possess anti-viral activity against the white spot syndrome virus, yet the issue remains unsolved. The present study was carried out to determine the anti-viral activity of methyl 1-chloro-7-methyl-2-propyl-1h-benzo[d] imidazole-5-carboxylate (C₁₃H₁₅N₂O₂Cl) against WSSV. The anti-viral activity of the synthetic compound was determined in freshwater crabs. Crabs were divided into three different experimental groups: healthy control groups (N.C.) received NTE buffer, positive control group (P.C.) crabs received WSSV, and treatment group crabs received WSSV along with synthetic weight compound. Experimental groups were observed for 30 days post-infection. Three different organs (gills, muscles, and head soft tissue (HST)) were dissected from all three groups and analyzed using molecular-based techniques, including polymerase chain reaction (PCR), Western blot, and histopathology. Clinical signs of WSSV were observed in the positive and N.C. groups; however, the treatment group showed a 100% survival rate. Confirmation was done using PCR, Western blot, and histopathology. These results demonstrated that the given synthetic compound has significant anti-viral activity against WSSV.

  相似文献   

Micronutrients (Fe,Mn, Zn and Cu) balance under long-term application of fertilizer and manure in a tropical rice-rice system     

Mohammad Shahid  Arvind Kumar Shukla  Pratap Bhattacharyya  Rahul Tripathi  Sangita Mohanty  Anjani Kumar  Banwari Lal  Priyanka Gautam  Rajagounder Raja  Bipin Bihari Panda  Brundaban Das  Amaresh Kumar Nayak 《Journal of Soils and Sediments》2016,16(3):737-747

  相似文献   

Sequence analysis of Toll-like receptor genes 1-10 of goat (Capra hircus)     

Raja A  Vignesh AR  Mary BA  Tirumurugaan KG  Raj GD  Kataria R  Mishra BP  Kumanan K 《Veterinary immunology and immunopathology》2011,140(3-4):252-258

This study involved cloning and sequencing of the coding regions of all 10 Toll-like receptor (TLR) genes of goat. Goat TLR 1-10 gene sequences revealed a high degree of nucleotide identity with sheep and cattle sequences (>90%) and 75-85% with pig, mouse and human sequences. At the amino acid level, 85-99% similarity was observed with sheep and cattle and 60-85% with pig, mouse and human. TLR9c DNA of goat showed the highest amino acid identity to that of sheep (99%) while TLR8 cDNA showed the lowest identity of 88.7% to that of sheep. Variations were seen in the number of leucine rich repeats (LRRs) of goat TLRs as compared to other ruminant species with maximum differences in the TLR3 gene. Phylogenetic analysis through molecular evolution and genetic analysis (MEGA) software and multi dimensional scaling revealed a high degree of conservation of goat TLRs with those from other species. However when the TIR domain of all the TLRs were compared, goat TLR7 TIR alone showed a high divergence of 19.3 as compared to sheep sequences. This is the first report of the full-length cDNA sequences of all the 10 TLR genes of goats which would be a useful tool for the study of evolutionary lineages and for phylogenetic analysis.  相似文献   

Poor maternal nutrition inhibits muscle development in ovine offspring     

Sarah A Reed  Joseline S Raja  Maria L Hoffman  Steven A Zinn  Kristen E Govoni 《畜牧与生物技术杂志(英文版)》2015,(1):79-89

Background:Maternal over and restricted nutrition has negative consequences on the muscle of offspring by reducing muscle fiber number and altering regulators of muscle growth.To determine if over and restricted maternal nutrition affected muscle growth and gene and protein expression in offspring,36 pregnant ewes were fed 60%,100%or 140%of National Research Council requirements from d 31 ± 1.3 of gestation until parturition.Lambs from control-fed(CON),restricted-fed(RES) or over-fed(OVER) ewes were necropsied within 1 d of birth(n = 18) or maintained on a control diet for 3 mo(n = 15).Semitendinosus muscle was collected for immunohistochemistry,and protein and gene expression analysis.Results:Compared with CON,muscle fiber cross-sectional area(CSA) increased in RES(58%) and OVER(47%)lambs at 1 d of age(P 0.01);however at 3 mo,CSA decreased 15%and 17%compared with CON,respectively(P 0.01).Compared with CON,muscle lipid content was increased in OVER(212.4%) and RES(92.5%) at d 1(P 0.0001).Muscle lipid content was increased 36.1%in OVER and decreased 23.6%in RES compared with CON at 3mo(P 0.0001).At d 1,myostatin mRNA abundance in whole muscle tended to be greater in OVER(P = 0.07) than CON.Follistatin mRNA abundance increased in OVER(P = 0.04) and tended to increase in RES(P = 0.06) compared with CON at d 1.However,there was no difference in myostatin or follistatin protein expression(P 0.3).Phosphorylated Akt(ser473) was increased in RES at 3 mo compared with CON(P = 0.006).Conclusions:In conclusion,maternal over and restricted nutrient intake alters muscle lipid content and growth of offspring,possibly through altered gene and protein expression.  相似文献   

Improved model for studying hydrological process in the field-to-field irrigation system     

Ebadi  Arash  Raja  Omid  Ebrahimian  Hamed  Yazdani  Mohammad Reza  Rezaverdinejad  Vahid 《Paddy and Water Environment》2022,20(1):137-150

Paddy and Water Environment - One of the common irrigation systems in the paddy fields is field-to-field irrigation. The management of these irrigation systems is difficult and essential because of...  相似文献   

Recent Insights into Signaling Responses to Cope Drought Stress in Rice   总被引：1，自引：0，他引：1  

Muhammad Mahran Aslam  Muhammad Abdul Rehman Rashid  Mohammad Aquil Siddiqui  Muhammad Tahir Khan  Fozia Farhat  Shafquat Yasmeen  Imtiaz Ahmad Khan  Shameem Raja  Fatima Rasool  Mahboob Ali Sial  Zhao Yan 《水稻科学》2022,29(2):105-117

  相似文献